Detection of Metastatic Lung Cancer by Immunocytochemistry and Flow Cytometry in a Sample of Pleural Fluid

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Manoela Lira Reis
Rafaela Pilon
Aline Costa
Teresa Maria da Silva Figueiredo
Alisson Bigolin
Ana Carolina Rabello de Moraes
Renata Cristina Messores Rudolf de Oliveira
Maria Cláudia Santos da Silva

Abstract

Malignant pleural effusions (MPE) are common complications in cancer patients and indicate the spread of the primary disease (metastasis). For over a century, malignant effusion has been diagnosed through the observation of changes in the cavity effusion cells, such as the gold-standard cytomorphological analysis. Studies show that the multiparameter flow cytometric analysis is sensitive and rapid and allows for the immunophenotypic evaluation of a large number of cells. This report describes the case of a 64-year-old man diagnosed with lung cancer who had never had any kind of treatment‏ for this disease. The pleural sample was analyzed by morphological (quantitative and differential cytology) and immunocytochemical analyses. As a new tool for diagnosis of malignant pleural effusion, the sample was also analyzed by flow cytometry. In the case report described, flow cytometry was an effective and quick method for detecting neoplastic cells in pleural fluids. 

Keywords:
Malignant pleural effusions, new tool for diagnosis, flow cytometry.

Article Details

How to Cite
Reis, M., Pilon, R., Costa, A., Figueiredo, T. M., Bigolin, A., de Moraes, A., Rudolf de Oliveira, R., & da Silva, M. (2016). Detection of Metastatic Lung Cancer by Immunocytochemistry and Flow Cytometry in a Sample of Pleural Fluid. International Journal of Medical and Pharmaceutical Case Reports, 7(3), 1-5. https://doi.org/10.9734/IJMPCR/2016/26726
Section
Case Study