Detection of Metastatic Breast Carcinoma Cells in Bone Marrow by Flow Cytometry

Main Article Content

Manoela Lira Reis
Daniella Serafin Couto Vieira
Laura Otto Walter
Maria Claudia Santos-Silva

Abstract

Breast cancer is the most common cause of cancer death in women worldwide. Cytological, histological, and immunohistochemical techniques are routine laboratory tests for determining tumor subtypes. Over the past few years, laboratory diagnostic tests for breast cancer have become more complex, sophisticated, and specialized. This report describes the case of a young patient with metastatic breast cancer whose diagnosis was based on flow cytometric analysis of bone marrow aspirate. Flow cytometry showed to be an important tool in cancer diagnosis. Its application as a routine laboratory test for the diagnosis of solid tumors, such as breast cancer, can help provide fast results while increasing diagnostic coverage.

Keywords:
Breast carcinoma, bone marrow, flow cytometry, breast cancer.

Article Details

How to Cite
Reis, M., Vieira, D. S., Walter, L., & Santos-Silva, M. (2019). Detection of Metastatic Breast Carcinoma Cells in Bone Marrow by Flow Cytometry. International Journal of Medical and Pharmaceutical Case Reports, 12(3), 1-6. https://doi.org/10.9734/ijmpcr/2019/v12i330105
Section
Case Study

References

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394-424.

Winters S, Martin C, Murphy D, Shokar NK. Breast cancer epidemiology, prevention, and screening. Prog. Mol. Biol. Transl Sci. 2017;151:1-32.

Jafari SH, Saadatpour Z, Salmaninejad A, Momeni F, Mokhtari M, Nahand JS, Rahmati M, Mirzaei H, Kianmehr M. Breast cancer diagnosis: Imaging techniques and biochemical markers. J. Cell Physiol. 2018;233(7):5200-5213.

Migowski A, Dias MBK, Nadanovsky P, Silva GA, Sant’Ana DR, Stein AT. Guidelines for early detection of breast cancer in Brazil. III – Challenges for implementation. Cad Saúde Pública. 2018; 34(6).

Klarenbach S, Sims-Jones N, Lewin G, Singh H, Thériault G , Tonelli M, Doull M, Courage S, Garcia AJ, Thombs BD. Recommendations on screening for breast cancer in women aged 40–74 years who are not at increased risk for breast cancer. CMAJ. 2018;10;190(49).

Rojas K, Stuckey A. Breast cancer epidemiology and risk factors. Clin. Obstet Gynecol. 2016;59(4):651-672.

Li X, Zhang Y, Meisel J, Jiang R, Behera M, Peng L. Validation of the newly proposed American Joint Committee on Cancer (AJCC) breast cancer prognostic staging group and proposing a new staging system using the National Cancer Database. Breast Cancer Res Treat. 2018; 171(2):303-313.

Wang D, Xu J, Shi G, Yin G. Molecular markers′ progress of breast cancer treatment efficacy. J. Cancer Res Ther. 2015;11(Suppl 1):C11-5.

Swerdlow SH, Campo E, Harris NL, Jasse, E, Pireli SA, Stein H, Thiele J, Vardiman, JW. WHO classification of tumours of hermatopoietic and lymphoid tissues. 4th Ed. Lyon: IARC; 2017.

Betters DM. Use of flow cytometry in clinical practice. J. Adv. Pract. Oncol. 2015;6:435–440.

Adan A, Alizada G, Kiraz Y, Baran Y, Nalbant A. Flow cytometry: Basic principles and applications. Crit Rev Biotechnol. 2017;37(2):163-176.

Bhagwat N, Dulmage K, Pletcher CHJ, Wang L, Demuth W, Sen M, Balli1 D, Yee SS, Silin Sa, Tong F, Yu L, Moore JS, Stanger BZ, Dixon EP, Carpenter EL. An integrated fow cytometry based platform for isolation and molecular characterization of circulating tumor single cells and clusters. Sci Rep. 2018;8(1):5035.

Handoo A, Dadu T. Flow cytometry in pediatric malignancies. Indian Pediatrics. 2018;55(1):55-62.

Chernysheva O, Markina I, Demidov L, Kupryshina N, Chulkova S, Palladina A, Antipova A, Tupitsyn N. Bone marrow involvement in melanoma. Potentials for detection of disseminated tumor cells and characterization of their subsets by flow cytometry. Cells. 2019; 8(6):627.